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FREEZE-PRESERVATION OF HUMAN GRANULOCYTES WITH DIMETHYL SULFOXIDE AND HYDROXYETHYL STARCH: IS IT A GOOD TECHNIQUE.

Author(s) : LOPEZ M.

Type of article: Article

Summary

THE EFFECT OF DMSO AND HES ON VIABILITY AND FUNCTIONAL CAPACITY OF HUMAN GRANULOCYTES WERE ASSESSED AFTER INCUBATION OF GRANULOCYTES WITH CRYOADDITIVES AT 277 K (4 DEG C). RESULTS SHOWED THAT A CONCENTRATION OF DMSO HIGHER THAN 6% WAS TOXIC FOR GRANULOCYTES AFTER AN INCUBATION TIME OF 15 MINUTES, WHILE HES EXHIBITED NO TOXICITY AT CONCENTRATIONS AS HIGH AS 15%. HUMAN GRANULOCYTES CRYOPRESERVED WITH 5% DMSO AND 5% HES SHOWED A GOOD RECOVERY OF VIABLE CELLS, BUT ON THE CONTRARY A SIGNIFICANT IMPAIRMENT OF THEIR FUNCTIONAL CAPACITY. IN PARTICULAR, FROZEN-THAWED GRANULOCYTES QUICKLY LOST THEIR VIABILITY WHEN THEY WERE WASHED AND STORED AT 310 K (37 DEG C).

Details

  • Original title: FREEZE-PRESERVATION OF HUMAN GRANULOCYTES WITH DIMETHYL SULFOXIDE AND HYDROXYETHYL STARCH: IS IT A GOOD TECHNIQUE.
  • Record ID : 1985-1748
  • Languages: English
  • Source: Cryo-Letters - vol. 5 - n. 6
  • Publication date: 1984
  • Document available for consultation in the library of the IIR headquarters only.

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