ShareIN VIVO DISTRIBUTION OF CRYOGENICALLY PRESERVED GUINEA PIG GRANULOCYTES.
Author(s) : LIONETTI F. J.
Type of article: Article
Summary
GRANULOCYTES WERE ISOLATED FROM WHOLE BLOOD OF GUINEA PIGS BY COUNTERFLOW CENTRIFUGATION AND LABELED. ONE HALF WAS CRYOGENICALLY PRESERVED WITH 5% DIMETHYL SULFOXIDE (DMSO), 6% HYDROXYETHYL STARCH (HES), AND 4% HUMAN ALBUMIN, AT A RATE OF 4 K (4 C) PER MINUTE BY STORAGE AT 193 K (-80 DEG C) AND THEN STORED FOR 3 DAYS AT 193 K. THE FROZEN -THAWED -WASHED -RESUSPENDED LABELED GRANULOCYTES WERE USED FOR EXPERIMENTS ON LABORATORY ANIMALS.
Details
- Original title: IN VIVO DISTRIBUTION OF CRYOGENICALLY PRESERVED GUINEA PIG GRANULOCYTES.
- Record ID : 1981-0308
- Languages: English
- Source: Cryobiology - vol. 17 - n. 1
- Publication date: 1980/02
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Indexing
- Themes: The influence of refrigeration on cells, tissues and organs
- Keywords: Starch; Cryoprotectant; Laboratory; DMSO; Animal; Albumin; Blood; Granulocyte; Cryopreservation
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GRANULOCYTIC CRYOPRESERVATION: FURTHER STUDIES ...
- Author(s) : MCCARTHY D. M.
- Date : 1984
- Languages : English
- Source: Br. J. Haematol. - 56; 45-54; 5 tabl.; 26 ref. (voir aussi anal. 82-1052).
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FREEZE-PRESERVATION OF HUMAN GRANULOCYTES WITH ...
- Author(s) : LOPEZ M.
- Date : 1984
- Languages : English
- Source: Cryo-Letters - vol. 5 - n. 6
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EFFECT OF DIMETHYL SULPHOXIDE/HYDROXYETHYL STAR...
- Author(s) : SCHEIWE M. W., KORBER C., ENGLICH S.
- Date : 1984
- Languages : English
- Source: Cryo-Letters - vol. 5 - n. 5
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IMPROVED FUNCTIONAL RECOVERY OF HUMAN GRANULOCY...
- Author(s) : HILL R. S., STILL B. J., MACKINDER C. A.
- Date : 1981/12
- Languages : English
- Source: Cryobiology - vol. 18 - n. 6
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CRYOPRESERVATION OF HUMAN GRANULOCYTE-MACROPHAG...
- Author(s) : REINERS B., ZINTL F., PLENERT W.
- Date : 1986
- Languages : English
- Source: Cryo-Letters - vol. 7 - n. 5
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