High survival rate of one-cell mouse embryos cooled rapidly to -196 deg C after exposure to a propylene glycol-DMSO-sucrose solution.
Author(s) : ABBEEL E. van den, ELST J. van der, LINDEN M. van der, STEIRTEGHEM A. C. van
Type of article: Article
Summary
When one-cell mouse embryos were exposed for 3 min to a solution containing 2.25 molpropylene glycol-2.25 mol DMSO-0.25 mol sucrose before rapid cooling to -196 deg C, 98% of the zygotes were morphologically intact after rapid thawing.
Details
- Original title: High survival rate of one-cell mouse embryos cooled rapidly to -196 deg C after exposure to a propylene glycol-DMSO-sucrose solution.
- Record ID : 1998-1291
- Languages: English
- Source: Cryobiology - vol. 34 - n. 1
- Publication date: 1997/02
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- Date : 1990/09
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- Author(s) : SHAW J. M., TROUNSON A. O.
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- Date : 1993/05
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- Source: Cryobiology - vol. 32 - n. 1
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- Author(s) : FAHNING M. L., GARCÍA M. A.
- Date : 1992/02
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- Source: Cryobiology - vol. 29 - n. 1
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