ShareStatus of cryopreservation of embryos from domestic animals.
Author(s) : FAHNING M. L., GARCÍA M. A.
Type of article: Article
Summary
Glycerol was used as a cryoprotectant for spermatozoa and then for embryos; this was successful for several animals, but not for swine; freezing techniques are described, using an "equilibrium method" with a solution of glycerol, cooled and frozen relatively slowly till -30 deg C and then rapidly with liquid nitrogen. There is also a nonequilibrium method in which a special solution (sucrose, glycerol and propylene glycol) allows a rapid vitrification of the cells and embryos, giving better results in some cases.
Details
- Original title: Status of cryopreservation of embryos from domestic animals.
- Record ID : 1993-0539
- Languages: English
- Source: Cryobiology - vol. 29 - n. 1
- Publication date: 1992/02
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