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Hypothermic preservation of human oocytes with antifreeze proteins from sub-polar fish.

Author(s) : ITSKOVITZ-ELDOR J., LEVRON J., ARAV A., AMI S. bar-, STEIN D. W., FLETCHER G. L., RUBINSKY B.

Type of article: Article

Summary

Mature human oocytes (n=29) were preserved at 4 deg C for 20 hours in phosphate buffer solution and in phosphate buffer solution with various concentrations of antifreeze proteins isolated from the winter flounder or the ocean pout. Fertilization and early embryo cleavage rates were increased by the addition of antifreeze proteins 1 milligram/milliliter and reduced when the concentration of antifreeze proteins was increased to 10 milligram/milliliter. These preliminary results are consistent with earlier animal studies and with the known ability of antifreeze proteins to stabilize membranes at hypothermic temperatures.

Details

  • Original title: Hypothermic preservation of human oocytes with antifreeze proteins from sub-polar fish.
  • Record ID : 1994-1254
  • Languages: English
  • Source: Cryo-Letters - vol. 14 - n. 4
  • Publication date: 1993/07
  • Document available for consultation in the library of the IIR headquarters only.

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