Cultured human endothelial cells as a model for hypothermic preservation.

Author(s) : HIDALGO M. A., AMBROSE I. J., GODDARD J. G., FULLER B. J., GREEN C. J.

Type of article: Article

Summary

Human vein endothelial cell cultures were subjected to 3, 8, 16 or 24 hours of normoxic hypothermia (0-4 deg C) in either culture medium or one of two organ preservation solutions containing low or high levels of potassium. Cold storage altered endothelial cell shape in all storage groups. The damage increased with the length of the cold insult, most markedly between 8 and 16 hours of storage. Neither of the solutions tested improved the appearance or viability of cells compared with those stored in culture medium and subjected to normoxic hypothermia.

Details

  • Original title: Cultured human endothelial cells as a model for hypothermic preservation.
  • Record ID : 1994-1854
  • Languages: English
  • Source: Cryo-Letters - vol. 14 - n. 6
  • Publication date: 1993/11
  • Document available for consultation in the library of the IIR headquarters only.

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