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IMPROVED HISTOLOGICAL PROCEDURE FOR MICROSCOPIC DEMONSTRATION OF RELATED CHANGES IN FISH MUSCLE TISSUE STRUCTURE DURING HOLDING AND FREEZING.

Author(s) : BELLO R. A., LUFT J. H., PIGOTT G. M.

Type of article: Article

Summary

A SIMPLE AND PRACTICAL HISTOLOGICAL PREPARATIVE PROCEDURE THAT CAUSES MINIMUM DAMAGE AND DISTORTION IN THE ARRANGEMENT AND ORGANIZATION OF MUSCULAR TISSUE OF FISH WAS DEVELOPED, WHICH INCLUDES: FIXATION IN SANFELICE FLUID, DEHYDRATION IN 2-METHOXYETHANOL, INFILTRATION IN NITROCELLULOSE, CLEARING IN CHLOROFORM, INFILTRATION AND EMBEDDING IN PARAFFIN, SECTIONING (4-8 MICROMETERS), AND STAINING IN A MODIFIED MASSON-TRICHROME STAIN. THE PROCEDURE PERMITTED THE CLEAR OBSERVATION OF MUSCLE CELLS AT THE MYOFIBRILLAR LEVEL. SENSIBLE CHANGES IN THE CONFIGURATION AND ARRANGEMENT OF THE MUSCULAR TISSUE DUE TO THE RATE OF FREEZING AND THE POSTMORTEM CONDITION, INCLUDING SPOILAGE, WERE CLEARLY DISTINGUISHED. HOLDING AND SPOILAGE PROGRESSIVELY EXPANDED THE EXTRACELLULAR SPACE AT THE EXPENSE OF SHRUNKEN MUSCLE CELLS AND CLUMPED MYOFIBRILS. FREEZING ALSO INCREASED THE EXTRACELLULAR SPACE WITH CELLULAR AND MYOFIBRILLAR COMPRESSION BETWEEN ICE CRYSTALS. THESE ALTERATIONS ARE DIAGNOSTIC OF PRIOR SUE TREATMENT AND ARE EASILY OBTAINED WITH SIMPLE HISTOLOGICAL METHODS.

Details

  • Original title: IMPROVED HISTOLOGICAL PROCEDURE FOR MICROSCOPIC DEMONSTRATION OF RELATED CHANGES IN FISH MUSCLE TISSUE STRUCTURE DURING HOLDING AND FREEZING.
  • Record ID : 1982-0155
  • Languages: English
  • Publication date: 1981
  • Source: Source: J. Food Sci.
    vol. 46; n. 3; 733-737; 740; 5 fig.; 3 tabl.; ref.
  • Document available for consultation in the library of the IIR headquarters only.

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