Summary
The effects of preculture on freezing resistance, sugar and water content, and the cell structure of asparagus shoot tips were examined. Freezing resistance of tips was increased by a 48 hour preculture on a medium supplmented with a high concentration of sugar. The optimal concentration of sugar in the preculture medium was 0.5 M, regardless of the sugar. It is suggested that the uptake of sugars and dehydration occurred in cells during preculture, with consequent increase of freezing resistance of precultured shoot tips.
Details
- Original title: Increase in freezing resistance of excised shoot tips of Asparagus officinalis L. by preculture on sugar-rich media.
- Record ID : 1998-3198
- Languages: English
- Source: Cryobiology - vol. 34 - n. 3
- Publication date: 1997/05
Links
See other articles in this issue (7)
See the source
Indexing
- Themes: The influence of refrigeration on cells, tissues and organs
- Keywords: Plant (botany); Cryobiology; Treatment; Asparagus; Sugar; Shoot; Freezing
-
Experimental parameters underlying failure or s...
- Author(s) : BERJAK P., WALKER M., WATT M. P., et al.
- Date : 1999/07
- Languages : English
- Source: Cryo-Letters - vol. 20 - n. 4
View record
-
The pattern of freezing of grapevine shoots dur...
- Author(s) : HAMED F., FULLER M. P., TELLI G.
- Date : 2000/07
- Languages : English
- Source: CryoLetters - vol. 21 - n. 4
View record
-
A novel preculturing for enhancing the survival...
- Author(s) : MATSUMOTO T., SAKAI A., NAKO Y.
- Date : 1998/01
- Languages : English
- Source: Cryo-Letters - vol. 19 - n. 1
View record
-
Cryopreservation of pineapple (Ananas comosus) ...
- Author(s) : GONZÁLES-ARNAO M. T., MÁRQUEZ-RAVELO M., URRA-VILLAVICENCIO C., MARTINEZ-MONTERO M., ENGELMANN F.
- Date : 1998/11
- Languages : English
- Source: Cryo-Letters - vol. 19 - n. 6
View record
-
Cryopreservation of apices isolated from garlic...
- Author(s) : MAKOWSKA Z., KELLER J., ENGELMANN F.
- Date : 1999/05
- Languages : English
- Source: Cryo-Letters - vol. 20 - n. 3
View record