Induction of freeze-sensitive mutants from a freeze-tolerant yeast, Torulaspora delbrueckii.

Author(s) : MURAKAMI Y., HAHN Y. S., YOKOIGAWA K., ENDO K., KAWAI H.

Type of article: Article

Summary

Cryosensitive mutants are induced through incubation with ethyl-methane sulfonate as mutagenic agent. An optimal mutation rate is reached through incubation at 30 deg C for 75 minutes. Cryotolerance of the mutants is evaluated by measuring their fermentation activity before and after freezing in a liquid or paste medium. The 60B3 strain is the most cryosensitive.

Details

  • Original title: Induction of freeze-sensitive mutants from a freeze-tolerant yeast, Torulaspora delbrueckii.
  • Record ID : 1995-1212
  • Languages: English
  • Source: Biosci. Biotechnol. Biochem. - vol. 58 - n. 1
  • Publication date: 1994

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