ShareInduction of freeze-sensitive mutants from a freeze-tolerant yeast, Torulaspora delbrueckii.
Author(s) : MURAKAMI Y., HAHN Y. S., YOKOIGAWA K., ENDO K., KAWAI H.
Type of article: Article
Summary
Cryosensitive mutants are induced through incubation with ethyl-methane sulfonate as mutagenic agent. An optimal mutation rate is reached through incubation at 30 deg C for 75 minutes. Cryotolerance of the mutants is evaluated by measuring their fermentation activity before and after freezing in a liquid or paste medium. The 60B3 strain is the most cryosensitive.
Details
- Original title: Induction of freeze-sensitive mutants from a freeze-tolerant yeast, Torulaspora delbrueckii.
- Record ID : 1995-1212
- Languages: English
- Source: Biosci. Biotechnol. Biochem. - vol. 58 - n. 1
- Publication date: 1994
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