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INFLUENCE OF TREHALOSE ON THE PHASE STRUCTURE AND MECHANISM OF PHASE TRANSITIONS OF HYDRATED AND FREEZE-DRIED DIPALMITOYLPHOSPHATIDYLCHOLINE (DPPC) MULTILAYERS. X-RAY DIFFRACTION AND DIFFERENTIAL SCANNING CALORIMETRY STUDIES.

Author(s) : TENCHOV B. G.

Summary

TREHALOSE ENTERS THE AQUEOUS SPACES OF HYDRATED MULTILAYERS AND INCREASES INTERBILAYER SEPARATION. IT DOES NOT AFFECT THE LIPID CHAIN PACKING AND ALSO THE SLOW ISOTHERMAL CONVERSION AT 277 K (4 DEG C) OF THE METASTABLE GEL PHASE INTO AN EQUILIBRIUM CRYSTALLINE PHASE. TREHALOSE STRONGLY DEPRESSES THE TRANSITION TEMPERATURES OF DPPC MULTILAYERS FREEZE-DRIED OR DESICCATED FROM AN INITIALLY FULLY HYDRATED STATE. THIS IS STRONGLY DEPENDENT ON THE INITIAL PHASE STATE OF THE LIPID MULTILAYERS PRIOR TO THEIR DRYING. IT IS SUGGESTED THAT TREHALOSE REDUCES THE INTERBILAYER HYDROGEN BOND COUPLING AND THUS MODIFIES THE THERMAL STABILITY AND PHASE TRANSITION, MECHANISM OF BILAYERS.

Details

  • Original title: INFLUENCE OF TREHALOSE ON THE PHASE STRUCTURE AND MECHANISM OF PHASE TRANSITIONS OF HYDRATED AND FREEZE-DRIED DIPALMITOYLPHOSPHATIDYLCHOLINE (DPPC) MULTILAYERS. X-RAY DIFFRACTION AND DIFFERENTIAL SCANNING CALORIMETRY STUDIES.
  • Record ID : 1988-2109
  • Languages: English
  • Publication date: 1987/08/02
  • Source: Source: Proc. 3rd natl. Sch. Cryobiol. Freeze-drying, Sofia
    105-115; 10 fig.; 35 ref.
  • Document available for consultation in the library of the IIR headquarters only.

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