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Intracellular ice formation of mouse osteoblasts during cryopreservation.

[In Chinese. / En chinois.]

Author(s) : LIU B. L., MCGRATH J., HUA Z.

Type of article: Article

Summary

Intracellular ice formation is one of the factors that causes cell damage during cryopreservation process. The study of intracellular ice formation will provide fundamental information for the development of appropriate cryopreservation protocols. The possibility of intracellular ice formation at various cooling rates for mouse osteoblasts was studied by using a cryomicoscopy system. The water transportation and ice nucleation parameters were used in the coupled water transport/ice nucleation model to predict the probability of intracellular ice formation over a wide range of cooling rates. The calculated probability of intracellular ice formation was in good agreement with the experimental data, suggesting the validity of the predicted parameters.

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Pages: 22-25

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Details

  • Original title: [In Chinese. / En chinois.]
  • Record ID : 2007-1307
  • Languages: Chinese
  • Source: Journal of Refrigeration - vol. 28 - n. 1
  • Publication date: 2007/02

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