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SUCCESSFUL IN VITRO FERTILIZATION OF MOUSE OOCYTES AFTER CRYOPRESERVATION USING GLYCEROL.

Author(s) : FULLER B. J., BERNARD A.

Type of article: Article

Summary

MATURE UNFERTILIZED OOCYTES COLLECTED FROM SUPEROVULATED MICE WERE FROZEN AND STORED AT 77 K (-196 DEG C) USING GLYCEROL AS CRYOPROTECTANT. THE CRYOPRESERVATION PROTOCOL USED WAS DESIGNED AFTER STUDYING PHOTOMICROGRAPHS OF CELL VOLUME CHANGES TAKEN AT 310 K (37 DEG C) DURING ADDITION AND REMOVAL OF CRYOPROTECTIVE ADDITIVE. NO SIGNIFICANT DIFFERENCE WAS FOUND BETWEEN THE FERTILIZATION RATES OF FROZEN OR UNFROZEN OOCYTES AFTER EQUILIBRATION WITH GLYCEROL.

Details

  • Original title: SUCCESSFUL IN VITRO FERTILIZATION OF MOUSE OOCYTES AFTER CRYOPRESERVATION USING GLYCEROL.
  • Record ID : 1985-1313
  • Languages: English
  • Source: Cryo-Letters - vol. 5 - n. 5
  • Publication date: 1984
  • Document available for consultation in the library of the IIR headquarters only.

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