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Damage caused by extracellular ice to attached osteoblast cells during cryopreservation.

[In Chinese. / En chinois.]

Author(s) : HAO B., LIU B., LIN P., et al.

Type of article: Article

Summary

Extracellular ice is one of the main factors that cause cell death in cryopreservation process. The damage caused by ice to the attached osteoblast cells was quantitatively studied using a directional ice formation cryostage. The results show that the cell recovery was 89.3 plus or minus 18.9% when the angle between ice forming direction and the attached cells was 0-30°, and the recovery of cells was 49.0 plus or minus 19.0% and 42.8 plus or minus 15.8% when the angle was 30-60 and 60-90°, respectively. These findings confirm that the mechanical contact area between the cells and the formed ice influences the recovery of cryopreserved cells.

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Pages: pp. 59-62

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