ShareISOLATION AND CRYOPRESERVATION OF HUMAN PERIPHERAL BLOOD MONOCYTES.
Author(s) : TSVETKOV T.
Type of article: Article
Summary
BUFFY COATS, SEPARATED AND POOLED TOGETHER, ARE USED FOR ISOLATION OF MONOCYTES. CELL DENSITY OF WORKING SUSPENSION IS INCREASED UP TO 650 MILLION CELLS/75 CM2 SURFACE BY MULTIPLICATION OF THE ACTIVE FIBRONECTIN SITES. FOR THE PURPOSE, CRYOPRECIPITATE IS USED FOR COATING THE GLASS-GELATIN SURFACE. MONOCYTES COULD BE SUCCESSFULLY CRYOPRESERVED WITH DMSO CRYOPROTECTIVE SOLUTION.
Details
- Original title: ISOLATION AND CRYOPRESERVATION OF HUMAN PERIPHERAL BLOOD MONOCYTES.
- Record ID : 1987-2023
- Languages: English
- Source: Cryobiology - vol. 23 - n. 6
- Publication date: 1986
Links
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Indexing
- Themes: The influence of refrigeration on cells, tissues and organs
- Keywords: Cryoprotectant; DMSO; Blood; Monocyte; Cryopreservation
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CRYOPRESERVATION OF HIGHLY PURIFIED HUMAN PERIP...
- Author(s) : TAKAHASHI T. A.
- Date : 1988
- Languages : Japanese
- Source: Low Temperature Medicine - vol. 14 - n. 4
View record
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VITRIFICATION OF HUMAN MONOCYTES.
- Author(s) : TAKAHASHI T.
- Date : 1986
- Languages : English
- Source: Cryobiology - vol. 23 - n. 2
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CRYOPRESERVATION OF ENUCLEATED HUMAN NEUTROPHIL...
- Author(s) : VOETMAN A. A., BOT A. A. M., ROOS D.
- Date : 1984
- Languages : English
- Source: Blood - vol. 63 - n. 1
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ALTERATION OF THE ELECTROPHORETIC MOBILITY OF H...
- Author(s) : SKRABUT E. M.
- Date : 1983
- Languages : English
- Source: Cryobiology - vol. 20 - n. 6
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GRANULOCYTIC CRYOPRESERVATION: FURTHER STUDIES ...
- Author(s) : MCCARTHY D. M.
- Date : 1984
- Languages : English
- Source: Br. J. Haematol. - 56; 45-54; 5 tabl.; 26 ref. (voir aussi anal. 82-1052).
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