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CRYOPRESERVATION OF HIGHLY PURIFIED HUMAN PERIPHERAL BLOOD MONOCYTES.

[In Japanese. / En japonais.]

Author(s) : TAKAHASHI T. A.

Type of article: Article

Summary

HUMAN PERIPHERAL BLOOD MONOCYTES WERE PURIFIED BY COUNTERFLOW CENTRIFUGAL ELUTRIATION OF BUFFY COATS OBTAINED FROM NORMAL VOLUNTEERS. THE PURITY OF MONOCYTES WAS ABOUT 95% AND CELLULAR VIABILITY WAS HIGHER THAN 98%. THOSE HIGHLY PURIFIED MONOCYTES WERE SUSPENDED IN A MEDIUM CONTAINING 7.5% DMSO AND 5% FETAL CALF SERUM (OR 2% HUMAN SERUM ALBUMIN), AND FROZEN IN A PROGRAMMING FREEZER AT 1.25 K/MIN TO 193 K (-80 DEG C), PLUNGED INTO LIQUID NITROGEN (LN2 ) AND STORED FOR VARIOUS PERIOD (7-180 DAYS). THE NUMERICAL RECOVERY OF CRYOPRESERVED MONOCYTES STORED FOR 180 DAYS IN LN2 WAS 89%. FURTHERMORE, CRYOPRESERVED MONOCYTES (7 DAYS IN LN2 ) COULD PRODUCE INTERLEUKIN-L AFTER THE STIMULATION WITH LIPOPOLYSACCHARIDE FOR 24 HOURS, AND THOSE CELLS BECAME CYTOTOXIC AGAINST TUMORIGENIC CELLS.

Details

  • Original title: [In Japanese. / En japonais.]
  • Record ID : 1989-2521
  • Languages: Japanese
  • Source: Low Temperature Medicine - vol. 14 - n. 4
  • Publication date: 1988
  • Document available for consultation in the library of the IIR headquarters only.

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