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Measurement of permeating levels of cryoprotectant during ovarian tissue cryopreservation using 1H-NMR spectroscopy in human and porcine ovaries.

Author(s) : THOMAS N., BUSZA A., COOPER A., PAYNTER S., FULLER B., SHAW R.

Type of article: Article

Summary

The current studies have shown that cryoprotectant permeation into both porcine and human ovarian tissue is incomplete using protocols reported in the literature. Given that ice formation within the tissue may cause a randomised disruption of follicle structure, higher effective cryoprotectant concentrations may be required to avoid this, which will necessitate alternative approaches, such as perfusion or selective injection, and NMR spectroscopy can be a valuable adjunct to such studies.

Details

  • Original title: Measurement of permeating levels of cryoprotectant during ovarian tissue cryopreservation using 1H-NMR spectroscopy in human and porcine ovaries.
  • Record ID : 1998-2565
  • Languages: English
  • Source: Cryo-Letters - vol. 18 - n. 3
  • Publication date: 1997/05
  • Document available for consultation in the library of the IIR headquarters only.

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