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CRYOPRESERVATION OF BLOOD PLATELETS.

LA CRYOCONSERVATION DES PLAQUETTES.

Author(s) : LOPEZ M., ELGHOUZZI M. H.

Type of article: Article

Summary

THIS STUDY INCLUDES THE FOLLOWING CHAPTERS: I. PRINCIPLES OF PLATELET FREEZING PROCESSES (DMSO AND GLYCEROL). II. TECHNICAL PARAMETERS OF FREEZING (TEMPERATURE DECREASE; STORAGE LIFE AT 193 AND 77 K (-80 AND -196C); EFFECT OF PLASTIC BAGS ON PLATELET PRESERVATION). III. CONTROLS IN VITRO (RESPONSE TO THE OSMOTIC SHOCK; PLATELET AGGLOMERATION; MORPHOLOGY IN PHASE CONTRAST AND ULTRASTRUCTURE IN ELECTRON MICROSCOPY). IV. TESTS IN VIVO (RECIRCULATION OF PLATELETS; STORAGE LIFE OF CRYOPRESERVED PLATELETS; SHORTENING OF BLEEDING TIME). V. CONCLUSIONS. FROM PRESENT RESULTS, CRYOPRESERVATION IN DMSO RESULTS IN PLATELETS WITH A QUALITY SLIGHTLY HIGHER THAN PLATELETS STORED IN GLYCEROL, IN SPITE OF SOME DRAWBACKS OF DMSO. IN FRANCE, THERE SEEMS TO BE A TREND TO GLYCEROLISED PLATELETS INJECTED WITHOUT WASHING AFTER THAWING, THEREFORE AVAILABLE IN A SHORTER TIME (10-15 MIN) THAN PLATELETS PRESERVED IN DMSO (45-60 MIN). HOWEVER THIS TIMUM POINT. FUTURE IMPROVEMENTS SHOULD BE DIRECTED TOWARDS THE USE OF NEW CRYOPROTECTANTS AND THE PRECISE STUDY OF THE INTRACELLULAR FREEZING PROCESS. THE CRYOPRESERVATION OF PHENOTYPED PLATELETS IS A CHOICE AID FOR PATIENTS SUFFERING FROM ANTIPLATELET ALLO-IMMUNATION. FROZEN PLATELETS HAVE ALSO THE ADVANTAGE OF BEING FREED FROM MOST LEUCOCYTES AND ERYTHROCYTES. G.G.

Details

  • Original title: LA CRYOCONSERVATION DES PLAQUETTES.
  • Record ID : 1982-0629
  • Languages: French
  • Source: Rev. fr. Transfus. Immuno-Hématol. - vol. 23 - n. 6
  • Publication date: 1980
  • Document available for consultation in the library of the IIR headquarters only.

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