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NUCLEATION AND GROWTH OF ICE IN DEEPLY UNDERCOOLED ERYTHROCYTES.

Author(s) : MATHIAS S. F., FRANKS F., TRAFFORD K.

Type of article: Article

Summary

IN ORDER TO DEFINE THE CATALYTIC SITES WHICH PROMOTE INTRACELLULAR FREEZING UNDER CONDITIONS OF EXTRACELLULAR UNDERCOOLING, THE AUTHORS STUDIED POSSIBLE FREEZE PROPAGATION BETWEEN DEEP UNDERCOOLED ERYTHROCYTES, THE NUCLEATION OF ICE IN ERYTHROCYTES GHOSTS, AND THE FREEZING BEHAVIOUR OF UNDERCOOLED HEMOGLOBIN SOLUTIONS. THE MAIN FINDINGS ARE: (1) NO CROSS-NUCLEATION OCCURS BETWEEN CELLS PACKED WITHIN THE SAME EMULSIFIED WATER DROPLET. (2) THE DSC (DIFFERENTIAL SCANNING CALORIMETRY) POWER-TIME CURVES OF INTACT ERYTHROCYTES AND GHOSTS ARE IDENTICAL. (3) THE NUCLEATION TEMPERATURE OF AQUEOUS HEMOGLOBIN, ISOLATED FROM THE CELLS, IS SUBSTANTIALLY LOWER THAN THAT OF WATER OR OF THE INTACT CYTOPLASM.

Details

  • Original title: NUCLEATION AND GROWTH OF ICE IN DEEPLY UNDERCOOLED ERYTHROCYTES.
  • Record ID : 1985-0820
  • Languages: English
  • Source: Cryobiology - vol. 21 - n. 2
  • Publication date: 1984

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