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Osmotic effects of dilution on erythrocytes after freezing and thawing in glycerol-containing buffer.

Author(s) : LOECKER R. de, GOOSSENS W., DUPPEN V. van, VERWILGHEN R., LOECKER W. de

Type of article: Article

Summary

Red blood cells frozen in 1.7 M and particularly in 2.2 M of glycerol retain a high degree of integrity upon thawing as long as the dilution procedure of the cryoprotectant is slow and preferentially compensated by the addition of sorbitol. As the non-penetrating cryoprotectant sorbitol induces initial cell shrinkage, cell swelling upon dilution of the cryoprotectants may not lead to hemolysis. However, rapid dilution of glycerol even with buffer containing up to 0.50 M of sorbitol cannot be achieved without provoking considerable hemolysis.

Details

  • Original title: Osmotic effects of dilution on erythrocytes after freezing and thawing in glycerol-containing buffer.
  • Record ID : 1994-1875
  • Languages: English
  • Source: Cryobiology - vol. 30 - n. 3
  • Publication date: 1993/06

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