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OSMOTIC EFFECTS OF RAPID DILUTION OF CRYOPROTECTANTS. 2. EFFECTS ON HUMAN ERYTHROCYTE HEMOLYSIS.

Author(s) : LOECKER R. de, PENNINCKX F.

Type of article: Article

Summary

EXPOSURE OF HUMAN RED BLOOD CELLS (RBC) TO DMSO FOLLOWED BY RAPID DILUTION RESULTS IN CONSIDERABLE HEMOLYSIS. THE REGISTERED MEAN CORPUSCULAR VOLUMES (MCV) VALUES PROVE TO BE THE RESULT OF AVERAGES BETWEEN NORMAL CELL VOLUMES AND RBC SPHERES ORIGINATING DURING DILUTION. BY SIMULTANEOUS EXPOSURE OF THE CELLS TO DMSO AND TO THE NONPENETRATING SORBITOL THE MCV PRIOR TO DILUTION IS REDUCED TO UP TO AROUND 50% OF THE ORIGINAL VOLUMES. CELL SWELLING OCCURRING UPON RAPID DILUTION OF THE CRYOPROTECTANTS WILL THUS NOT REACH DILUTION HEMOLYSIS LEVELS.

Details

  • Original title: OSMOTIC EFFECTS OF RAPID DILUTION OF CRYOPROTECTANTS. 2. EFFECTS ON HUMAN ERYTHROCYTE HEMOLYSIS.
  • Record ID : 1988-0388
  • Languages: English
  • Source: Cryo-Letters - vol. 8 - n. 3
  • Publication date: 1987
  • Document available for consultation in the library of the IIR headquarters only.

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