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OSMOTIC EFFECTS OF RAPID DILUTION OF CRYOPROTECTANTS. 1. EFFECTS ON HUMAN ERYTHROCYTE SWELLING.

Author(s) : LOECKER R. de, PENNINCKX F., KERREMANS R.

Type of article: Article

Summary

INITIAL EXPOSURE OF HUMAN RED BLOOD CELLS TO DMSO FOLLOWED BY RAPID DILUTION OF THE PERMEATING CRYOPROTECTANT RESULTS IN CONSIDERABLE CELL SWELLING WHICH CAN BE REDUCED BY SIMULTANEOUS EXPOSURE TO THE NONPENETRATING SORBITOL. IN ORDER TO ACHIEVE OPTIMAL RECUPERATION, THE ELECTROLYTE COMPOSITION OF THE EXPOSURE BUFFERS SHOULD ESSENTIALLY BE PHOSPHATE BUFFERED CONTAINING LOW SODIUM IONS AND HIGH POTASSIUM IONS CONCENTRATIONS.

Details

  • Original title: OSMOTIC EFFECTS OF RAPID DILUTION OF CRYOPROTECTANTS. 1. EFFECTS ON HUMAN ERYTHROCYTE SWELLING.
  • Record ID : 1988-0387
  • Languages: English
  • Source: Cryo-Letters - vol. 8 - n. 3
  • Publication date: 1987
  • Document available for consultation in the library of the IIR headquarters only.

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