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OSMOTIC EFFECTS OF RAPID DILUTION OF CRYOPROTECTANTS ON ERYTHROCYTE SWELLING AND HEMOLYSIS.

[In Russian. / En russe.]

Author(s) : LOECKER R. de, PENNINCKX F.

Type of article: Article

Summary

CELL SWELLING AND HEMOLYSIS OF RED BLOOD CELLS RESULTING FROM DILUTION OF THE PENETRATING CRYOPROTECTANT DMSO CAN BE REDUCED BY THE SIMULTANEOUS ADDITION OF THE NONPENETRATING SORBITOL DURING THE EXPOSURE PERIOD. THE SIMULTANEOUS ADDITION OF SORBITOL DURING EXPOSURE REDUCES THE INITIAL CELL VOLUMES, THUS AVOIDING HEMOLYSIS LEVELS BEING REACHED DURING THE REMOVAL OF CRYOPROTECTANTS. PHOSPHATE BUFFERED SOLUTIONS CHARACTERIZED BY AN INTRACELLULAR ELECTROLYTE COMPOSITION PROVIDE OPTIMAL PROTECTION AGAINST EXCESSIVE CELL SWELLING.

Details

  • Original title: [In Russian. / En russe.]
  • Record ID : 1990-0412
  • Languages: Russian
  • Source: Kriobiologija - n. 3
  • Publication date: 1989
  • Document available for consultation in the library of the IIR headquarters only.

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