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MEASUREMENT AND MODELLING OF THE OSMOTIC BEHAVIOUR OF CELLS DURING FREEZING.

[In Russian. / En russe.]

Author(s) : DILLER K. R.

Type of article: Article

Summary

COMPLEMENTARY EXPERIMENTAL AND ANALYTICAL METHODS WERE A TO QUANTIFY THE OSMOTIC PROPERTIES OF LIVING CELLS AT SUBZERO TEMPERATURES. SUSPENSIONS OF SUBJECT CELLS ARE FROZEN AND THAWED AT COMPUTER PROGRAMMED AND CONTROLLED RATES ON A CRYOMICROSCOPE. DEFINED OSMOTIC STRESS IS PRODUCED IN THE CELLS BY EXPOSURE TO EXTRACELLULAR ICE AT A CONSTANT SUBZERO TEMPERATURE. A NETWORK THERMODYNAMIC MODEL FOR THE FREEZING PROCESS IS FIT TO THE TRANSIENT VOLUME DATA TO DETERMINE AN APPARENT PERMEABILITY OF THE PLASMA MEMBRANE TO WATER.

Details

  • Original title: [In Russian. / En russe.]
  • Record ID : 1991-0418
  • Languages: Russian
  • Source: Kriobiologija - n. 1
  • Publication date: 1990
  • Document available for consultation in the library of the IIR headquarters only.

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