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PLATELET CRYOPRESERVATION WITH GLYCEROL, DEXTRAN, AND MANNITOL: RECOVERY OF 5-HYDROXYTRYPTAMINE UPTAKE AND HYPOTONIC STRESS RESPONSE.

Author(s) : BRODTHAGEN U. A., ARMITAGE W. J., PARMAR N.

Type of article: Article

Summary

HUMAN PLATELETS WERE FROZEN IN 0.5 M GLYCEROL, 0.5 M GLYCEROL + 3% DEXTRAN T 40, OR 0.5 M GLYCEROL + 5% MANNITOL. THE RECOVERY OF ACTIVE TRANSPORT OF 5-HT AND THE HYPOTONICSTRESS RESPONSE AFTER FREEZING WERE DEPENDENT ON THE RATE OF COOLING: THE OPTIMUM RANGE OF RATES WAS BETWEEN 12 AND 23 K/MIN. THE NUMERICAL RECOVERY OF CELLS WAS INDEPENDENT OF COOLING RATE, BUT FREEZING ALTERED THE CELL-SIZE DISTRIBUTION. THE COMBINATION OF DEXTRAN AND GLYCEROL WAS NO BETTER THAN GLYCEROL ALONE. MANNITOL ADVERSELY AFFECTED PLATELET 5-HT UPTAKE AND THIS WAS REFLECTED IN A LOW RECOVERY.

Details

  • Original title: PLATELET CRYOPRESERVATION WITH GLYCEROL, DEXTRAN, AND MANNITOL: RECOVERY OF 5-HYDROXYTRYPTAMINE UPTAKE AND HYPOTONIC STRESS RESPONSE.
  • Record ID : 1985-2687
  • Languages: English
  • Source: Cryobiology - vol. 22 - n. 1
  • Publication date: 1985

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