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Open pulled straw (OPS) vitrification of porcine blastocysts: simple procedure yielding excellent in vitro survival, but so far no piglets following transfer.

Author(s) : HOLM P., VATJA G., MACHÁTY Z., et al.

Type of article: Article

Summary

In 2 trials, 346 porcine in vivo blastocysts were vitrified in heat-softened and pulled French straws by the PS vitrification method. After thawing, embryos were either cultured in vitro (n= 89) for 24 to 48 hours or transferred surgically (n= 257) to 11 synchronised recipients without further culture. In trial 1,88 and 71% of thawed blastocysts reexpanded and hatched in vitro, respectively, while 71% reexpanded in trial 2. However, no piglets were born following transfer, though 1 recipient was diagnosed pregnant at Day 56 of gestation.

Details

  • Original title: Open pulled straw (OPS) vitrification of porcine blastocysts: simple procedure yielding excellent in vitro survival, but so far no piglets following transfer.
  • Record ID : 2000-2800
  • Languages: English
  • Source: Cryo-Letters - vol. 20 - n. 5
  • Publication date: 1999/09
  • Document available for consultation in the library of the IIR headquarters only.

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