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Protection of freeze-dried Escherichia coli by trehalose upon exposure to environmental conditions.

Author(s) : ISRAELI E., SHAFFER B. T., LIGHTHART B.

Type of article: Article

Summary

Freeze-dried cultures of wild-type and genetically engineered strains of Escherichia coli lost their colony-forming ability upon exposure to air, visible light, and certain relative humidity levels. It was possible to protect both strains from these lethal effects with 100 milliM trehalose, a concentration calculated to just saturate the interphospholipid spaces in the cell membrane, thus preserving the liquid-crystalline structure. The trehalose protection increased viability as much as 2,000-4,000% over nontreated populations. In all cases, exposure to environmental conditions was more damaging to the genetically engineered strain.

Details

  • Original title: Protection of freeze-dried Escherichia coli by trehalose upon exposure to environmental conditions.
  • Record ID : 1994-1916
  • Languages: English
  • Source: Cryobiology - vol. 30 - n. 5
  • Publication date: 1993/10

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