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SITES OF FREEZING INJURY IN MAMMALIAN CELLS.

[In Russian. / En russe.]

Author(s) : MCGANN L. E., YANG H., WALTERSON M. L.

Type of article: Article

Summary

THE NATURE OF DAMAGE IMMEDIATELY AFTER THAWING WAS INVESTIGATED BY MONITORING SEVERAL FUNCTIONAL TESTS OF THE CELL AND THE PLASMA MEMBRANE. THREE CELL TYPES(HAMSTER FIBROBLASTS, HUMAN LYMPHOCYTES, AND GRANULOCYTES) WERE COOLED IN THE ABSENCE OF CRYOPROTECTIVE COMPOUND AT 1 K/MIN DOWN TO BETWEEN 263 AND 233 K (-10 AND -40 DEG C), THEN EITHER WARMED DIRECTLY IN WATER AT 310 K (37 DEG C) OR COOLED RAPIDLY TO 77 K (-196 DEG C) BEFORE RAPID WARMING. CELLS BEHAVED AS OSMOMETERS AFTER COOLING AT 1 K/MIN TO LOW TEMPERATURES AT WHICH THERE WERE NO RESPONSES MEASURED BY OTHER ASSAYS, DEMONSTRATING THAT THE PLASMA MEMBRANE RETAINEDITS SEMI-PERMEABLE PROPERTIES, AND IS THEREFORE NOT A PRIMARY SITE FOR INJURY SUSTAINED DURING SLOW COOLING.

Details

  • Original title: [In Russian. / En russe.]
  • Record ID : 1991-2316
  • Languages: Russian
  • Source: Kriobiologija - n. 3
  • Publication date: 1990
  • Document available for consultation in the library of the IIR headquarters only.

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