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SKIN BANKING METHODOLOGY: AN EVALUATION OF PACKAGE FORMAT, COOLING AND WARMING RATES, AND STORAGE EFFICIENCY.

Author(s) : RANDOLPH MAY S., DECLEMENT F. A.

Type of article: Article

Summary

THE TWO MAJOR SKIN PACKAGING FORMATS FOR TRANSPLANTABLE HUMAN SKIN, FLAT-FOLDED AND ROLLED-CYLINDRICAL, WERE EVALUATED WITH RESPECT TO THE CONTROL OF COOLING RATE, WARMING RATE, AND STORAGE EFFICIENCY. CONTRARY TO PREVIOUSLY PUBLISHED STATEMENTS, WHEN SKIN PACKAGED IN EITHER OF THE TWO FORMATS IS COOLED AT AN UNCONTROLLED RATE IN A LOW TEMPERATURE 203 K (-70 C) MECHANICAL REFRIGERATOR OR DRY-ICE CHEST, THE SMALLER SKIN DIMENSIONS COOL TOO RAPIDLY (UP TO -24 C MIN-1 ), WHILE THE PACKETS CONTAINING LARGER SKIN DIMENSIONS EXHIBIT PROLONGED EXOTHERMIC TEMPERATURE PLATEAUX (8-44 MIN), ALLOWING THE POSSIBILITY OF SIGNIFICANT CRYSTALLIZATION DAMAGE TO THE CELLS. ON THE OTHER HAND, CONTROLLED-RATE COOLING OF 1 KMIN-1 CAN BE OBTAINED USING A TEMPERATURE-FEEDBACK CONTROLLED-RATE FREEZER ALONG WITH A FLAT SKIN PACKET GEOMETRY. SKIN PROCESSED IN THE FLAT FORMAT IS CAPABLE OF BEING WARMED BY WATER IMMERSION ABOUT 10 TIMES MORE QUICKLY THAN ED FORMAT. THE FLAT FORMAT POSSESSES A TWO-TO THREEFOLD ADVANTAGE IN STORAGE EFFICIENCY. CAPITAL EQUIPMENT AND LIQUID NITROGEN USAGE FOR STORAGE IS DRASTICALLY DECREASED IF A FLAT PACKAGE FORMAT IS CHOSEN.

Details

  • Original title: SKIN BANKING METHODOLOGY: AN EVALUATION OF PACKAGE FORMAT, COOLING AND WARMING RATES, AND STORAGE EFFICIENCY.
  • Record ID : 1981-0288
  • Languages: English
  • Source: Cryobiology - vol. 17 - n. 1
  • Publication date: 1980/02

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