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Somatic cell cryopreservation and protoplast regeneration of important disease-resistant wild rice Oryza meyeriana Baill.

Type of article: Article

Summary

Oryza meyeriana Baill is reluctant in cell culture in vitro. In a series of experiments, callus with no regeneration ability was induced from young panicle of Oryza meyeriana. The callus was subcultured and propagated. Embryogenic cell clones were obtained after cryopreservation. Suspension cultures were established and protoplasts were isolated and regenerated into plants. Results of artificial inoculation of Xanthomonas campestris pv. Oryzae showed that the strong resistance did not change in the regenerated plants. The experiments demonstrated that cryopreservation of plant calli was a new way to obtain embryogenic cell line.

Details

  • Original title: Somatic cell cryopreservation and protoplast regeneration of important disease-resistant wild rice Oryza meyeriana Baill.
  • Record ID : 1999-3259
  • Languages: English
  • Source: Sci. China, C - vol. 41 - n. 4
  • Publication date: 1998/08
  • Document available for consultation in the library of the IIR headquarters only.

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