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STUDIES ON CELLULAR STRUCTURE AND ICE LOCATION IN FROZEN ORGANS AND TISSUES: THE USE OF FREEZE-SUBSTITUTION AND RELATED TECHNIQUES.

Author(s) : HUNT C. J.

Type of article: Article

Summary

RECENT STUDIES HAVE LED TO THE CONCLUSION THAT EXTRACELLULAR ICE PER SE CAN DAMAGE WHOLE ORGANS AND TISSUES. THUS INFORMATION ON THE AMOUNT AND DISTRIBUTION OF ICE IS AN IMPORTANT FACTOR IN THE DESIGN OF COOLING REGIMENS THAT AVOID INTRACELLULAR ICE FORMATION AND ATTEMPT TO LOCALIZE THE ICE FORMED IN AREAS OF THE TISSUE WHERE ITS DISRUPTIVE EFFECTS CAN BE MINIMIZED. FURTHERMORE, ULTRASTRUCTURAL STUDIES AT SUBFREEZING TEMPERATURES CAN ENHANCE THE INTERPRETATION OF INFORMATION GAINED FROM MORPHOLOGICAL AND FUNCTION STUDIES CONDUCTED BEFORE COOLING AND AFTER REWARMING. ISOTHERMAL FREEZE-FIXATION IS MOST SUITABLE FOR STUDIES CLOSE TO THE MELTING POINT, WHILE FREEZE-SUBSTITUTION CAN BE USED AT LOWER TEMPERATURES, EXTENDING AS FAR AS 153 K (-120 DEG C).

Details

  • Original title: STUDIES ON CELLULAR STRUCTURE AND ICE LOCATION IN FROZEN ORGANS AND TISSUES: THE USE OF FREEZE-SUBSTITUTION AND RELATED TECHNIQUES.
  • Record ID : 1985-1312
  • Languages: English
  • Source: Cryobiology - vol. 21 - n. 4
  • Publication date: 1984

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