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Survival of the cryptobiotic eutardigrade Adorybiotus coronifer during cooling to -196 deg C: effect of cooling rate, trehalose level, and short-term acclimation.

Author(s) : RAMLOV H., WESTH P.

Type of article: Article

Summary

The cryptobiotic eutardigrade Adorybiotus coronifer survives cooling to -196 deg C in the hydrated state, showing decreasing viability with faster cooling rates. The dissaccharide trehalose may have some cryoprotective effect. Cooling at 30 K/min to an intermediate temperature greater than the ice crystallization temperature of the sample before shock cooling to -196 deg C is lethal, whereas cooling at a rate of 30 K/min to an intermediate temperature less than the ice crystallization temperature of the sample before shock cooling to -196 deg C achieved a survival of about 55%. This is similar to the survival of animals cooled to -196 deg C at a constant rate of 30 K/min. The data support the hypothesis that the freeze tolerance exhibited by A. coronifer is closely related to its ability to be in the state of anhydrobiosis.

Details

  • Original title: Survival of the cryptobiotic eutardigrade Adorybiotus coronifer during cooling to -196 deg C: effect of cooling rate, trehalose level, and short-term acclimation.
  • Record ID : 1993-0518
  • Languages: English
  • Source: Cryobiology - vol. 29 - n. 1
  • Publication date: 1992/02

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