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THE ACTIVITY OF SOME ENZYMES AS A MARKER OF MEMBRANE INJURY UPON THE FREEZING OF ISOLATED MITOCHONDRIA.

Author(s) : IVANOV N., et al.

Summary

MITOCHONDRIA ISOLATED FROM THE LIVER OF MALE WISTAR RATS WERE FROZEN IN 0. 25 M SUCROSE AT 263 K (-10 C) AND KEPT AT THIS TEMPERATURE FOR 30 MIN. AFTER THAWING THE MITOCHONDRIA, THE ACTIVITY WAS STUDIED OF THE ENZYMES FIRMLY LINKED WITH THE MITOCHONDRIAL MEMBRANE, CYTOCHROME OXIDASE, NADH-CYTOCHROME-C-REDUCTASE AND SUCCINATE-DEHYDROGENASE, AND ALSO OF THE SOLUBLE MATRIX ENZYMES, MALATE DEHYDROGENASE AND GLUTAMATE DEHYDROGENASE. THE FREEZING OF ISOLATED MITOCHONDRIA RESULTS IN A RELIABLE DECREASE IN SUCCINATE DEHYDROGENASE ACTIVITY.

Details

  • Original title: THE ACTIVITY OF SOME ENZYMES AS A MARKER OF MEMBRANE INJURY UPON THE FREEZING OF ISOLATED MITOCHONDRIA.
  • Record ID : 1981-0271
  • Languages: English
  • Publication date: 1979/08/27
  • Source: Source: Proc. 25th eur. Meet. Meat Res. Work., Budapest
    vol. 1; n. 4.17; 253-259; 5 tabl.; 16 ref.
  • Document available for consultation in the library of the IIR headquarters only.

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