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The effect of freezing rate on the survival of cryopreserved African sharptooth catfish (Clarias gariepinus) spermatozoa.

Author(s) : STEYN G. J.

Type of article: Article

Summary

Cryoprotective agents were evaluated to find the optimal concentration of the cryoprotectant and most suitable combination of solution and cryoprotectant. A cryoprotective agent composed of 4% glucose and 9% glycerol yielded the best results. It was established that the optimal freezing rate is dependent on the composition of the cryoprotective agent. Maximal survival of catfish spermatozoa (60%) occurs at 5 deg C/minute and faster and slower freezing rates result in poor survival or no survival at all.

Details

  • Original title: The effect of freezing rate on the survival of cryopreserved African sharptooth catfish (Clarias gariepinus) spermatozoa.
  • Record ID : 1994-2561
  • Languages: English
  • Source: Cryobiology - vol. 30 - n. 6
  • Publication date: 1993/12

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