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The effect of polyvinylpyrrolidone and the cooling rate during corneal cryopreservation.

Author(s) : MADDEN P. W., TAYLOR M. J., HUNT C. J., PEGG D. E.

Type of article: Article

Summary

A method for cryopreserving rabbit cornea is discussed. A detailed protocol is presented with regard to cryoprotectants (DMSO, polyvinylpyrrolidone), isotonic medium, different cooling rates (0.03 to 125 deg C/minute). Loss of cell integrity after thawing is assessed, notably for endothelial cells. The results show that loss of cell integrity occurs at cooling rates greater than 1 deg C/minute, whereas at lower rates adhesion to the epithelial layer was impaired.

Details

  • Original title: The effect of polyvinylpyrrolidone and the cooling rate during corneal cryopreservation.
  • Record ID : 1994-1888
  • Languages: English
  • Source: Cryobiology - vol. 30 - n. 2
  • Publication date: 1993/04

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