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The effects of cryopreservation on protein synthesis and membrane transport in isolated rat liver mitochondria.

Author(s) : LOECKER P. de, FULLER B. J., LOECKER W. de

Type of article: Article

Summary

Protein synthesizing activity and membrane transport were examined in fresh and cryopreserved isolated rat liver mitochondria. In the presence of 0.6, 1.2 and 1.8 M final concentrations of DMSO, both metabolic parameters were considerably inhibited in the fresh samples and even more inhibited in the cryopreserved specimens. However, simple exposure to this penetrating cryoprotectant, followed by its subsequent removal by washing, did not seem to affect significantly the examined functions. Different freeze-thaw regimes were investigated, and results are given.

Details

  • Original title: The effects of cryopreservation on protein synthesis and membrane transport in isolated rat liver mitochondria.
  • Record ID : 1992-3066
  • Languages: English
  • Source: Cryobiology - vol. 28 - n. 5
  • Publication date: 1991/10

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