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THE HYDROLYSIS KINETICS OF P-NITROPHENYLACETATE IN FROZEN AQUEOUS SOLUTIONS CONTAINING CRYOPROTECTIVE ADDITIVES.

[In Russian. / En russe.]

Author(s) : GROMCENKO I. A.

Type of article: Article

Summary

PHYSICO-CHEMICAL PROPERTIES OF FROZEN AQUEOUS SYSTEMS WERE STUDIED BY THE KINETICS OF P-NITROPHENYLACETATE HYDROLYSIS. GLYCEROL WAS SHOWN TO STABILIZE PH, FAVOURING THE MAINTENANCE OF PHOSPHATE BUFFER SOLUTES. ON THE OTHER HAND, THE CRYOPROTECTANT ACTIVATED HYDROLYSIS, WHICH COULD LEAD TO A PARTIAL DEGRADATION OF BIOSTRUCTURES. THIS FACT SHOULD BE TAKEN INTO ACCOUNT WHEN DETERMINING THE TIME OF EQUILIBRATION OF BIOLOGICAL MATERIAL IN A CRYOPROTECTIVE MEDIUM BEFORE FREEZING.

Details

  • Original title: [In Russian. / En russe.]
  • Record ID : 1991-2311
  • Languages: Russian
  • Source: Kriobiologija - n. 3
  • Publication date: 1990
  • Document available for consultation in the library of the IIR headquarters only.

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