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Critical cooling rates for aqueous cryoprotectants in the presence of sugars and polysaccharides.

Author(s) : SUTTON R. L.

Type of article: Article

Summary

The technique of isothermal emulsion differential scanning calorimetry was used to determine time-temperature-transformation curves for aqueous glycerol and butane-2,3-diol in the presence of various polysaccharides and sugars. The critical cooling rate required to avoid the crystallization of ice in these solutions was then calculated. The polysaccharides used included starch hydrolysis products and dextrans of various molecular weights. The sugars used were sucrose, glucose, trehalose and raffinose. The results show that the critical cooling rates of butane-2,3-diol and glycerol are reduced by varying amounts by the addition of such materials but that reduction is not as great as is achieved by the addition of polyethylene glycol with a molecular weight of 400.

Details

  • Original title: Critical cooling rates for aqueous cryoprotectants in the presence of sugars and polysaccharides.
  • Record ID : 1994-1851
  • Languages: English
  • Source: Cryobiology - vol. 29 - n. 5
  • Publication date: 1992/10

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