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The influence of polyvinylpyrrolidone (PVP) on freezing of bovine in-vitro frozen blastocysts following biopsy.

Author(s) : SUZUKI T., SAHA S., SUMANTRI C., TAKAGI M., BOEDIONO A.

Type of article: Article

Summary

The viability and the percentage of embryos that developed to superior to 250 micrometers in diameter in the 5, 10, and 20% PVP groups were significantly higher than those that developed cryopreserved without PVP. Optimum development of in vitro culture of frozen-thawed biopsied blastocysts was obtained using 1.8 M ethylene glycol + 0.05 M trehalose and 20% PVP. Analysis of blastocysts superior to 250 micrometers in diameter showed that the number of inner cell mass cells of biopsied blastocysts cryopreserved in 1.8 M ethylene glycol + 0.05 M trehalose with or without PVP was not different from the number of unfrozen biopsied blastocysts. These results indicate that PVP has some beneficial effect on freezing of biopsied bovine blastocysts.

Details

  • Original title: The influence of polyvinylpyrrolidone (PVP) on freezing of bovine in-vitro frozen blastocysts following biopsy.
  • Record ID : 1996-2561
  • Languages: English
  • Source: Cryobiology - vol. 32 - n. 6
  • Publication date: 1995/12

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