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THE PRESERVATION OF ESCHERICHIA COLI HB 101 CULTURES CONTAINING A RECOMBINANT PLASMID WITH THE HUMAN DNA FRAGMENT.

Author(s) : GOLIMBET V. E., SIDJAKINA T. M., JAKOVLEV A. G.

Type of article: Article

Summary

THE INFLUENCE OF DIFFERENT PRESERVATION METHODS SUCH AS LYOPHILIZATION, FREEZING ON CARRIERS, AND CRYOPRESERVATION, ON THE VIABILITY AND GENETIC STABILITY OF E. COLI HB 101 CULTURES CONTAINING A RECOMBINANT PLASMID WITH THE HUMAN DNA FRAGMENT WAS STUDIED. GENETIC STABILITY WAS ESTIMATED BY MONITORING THE MAINTENANCE OF SELECTIVE MARKERS OF THE ANTIBIOTIC RESISTANCE AND STABILITY OF RESTRICTION SITES IN RECOMBINANT DNA. CRYOPRESERVATION AND STORAGE AT -196 DEG C APPEARED TO BE THE OPTIMUM METHOD FOR ENSURING HIGH VIABILITY AND GENETIC STABILITY.

Details

  • Original title: THE PRESERVATION OF ESCHERICHIA COLI HB 101 CULTURES CONTAINING A RECOMBINANT PLASMID WITH THE HUMAN DNA FRAGMENT.
  • Record ID : 1991-2313
  • Languages: Russian
  • Source: Kriobiologija/Cryobiology - 8 ref./vol. 28 - n. 2
  • Publication date: 1990

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