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THE ROLE OF GLYCEROL AND DMSO ON THE FREEZING OF FOWL SPERMATOZOA.

Author(s) : MAEDA T., TERADA T., TSUTSUMI Y.

Type of article: Article

Summary

TO CLARIFY THE PROTECTIVE MECHANISM OF CRYOPROTECTANTS IN FREEZING OF SPERMATOZOA, THE EFFECT OF GLYCEROL AND DMSO ON THE ULTRASTRUCTURE OF QUICK-FROZEN FOWL SPERMATOZOA WAS EXAMINED. IN CONTROL SAMPLES, FORMATION OF MANY LARGE ICE CRYSTALS WAS OBSERVED IN VARIOUS PARTS OF THE SPERMATOZOON. IN SAMPLES WITH GLYCEROL OR DMSO, NO FORMATION OF THE LARGE ICE CRYSTALS WAS FOUND BUT EXTENSIVE DISINTEGRATION OF THE PLASMA MEMBRANE WAS MORE OBVIOUS THAN IN THE CONTROL SAMPLES. THESE RESULTS SUGGEST THAT GLYCEROL AND DMSO PREVENT THE FORMATION OF LARGE ICE CRYSTALS DURING THE FREEZING PROCESS, AND THAT THIS PROTECTS THE INNER CYTOPLASMIC ORGANELLES, BUT MAY ADVERSELY AFFECT THE PLASMA MEMBRANE UNDER THE RAPID-FREEZING CONDITION.

Details

  • Original title: THE ROLE OF GLYCEROL AND DMSO ON THE FREEZING OF FOWL SPERMATOZOA.
  • Record ID : 1990-2152
  • Languages: English
  • Source: Cryo-Letters - vol. 10 - n. 6
  • Publication date: 1989/11
  • Document available for consultation in the library of the IIR headquarters only.

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