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The unique stability of the marsupial sperm acrosomal membranes examined by unprotected freeze-thawing and treatment with the detergent Triton X-100.

Author(s) : SISTINA Y., LIN M., MATE K. E., ROBINSON E. S., RODGER J. C.

Type of article: Article

Summary

Experiments were carried out on the acrosomes of spermatozoa of the tammar wallaby (Macropus eugenii), common brushtail possum (Trichosurus vulpecula) and grey short-tailed opossum (Monodelphis domestica). Light microscopy showed that 4% of opossum and 15% of opossum and wallaby spermatozoa lost their acrosomes after freeze-thawing. Electron microscopy revealed that freeze-thawing also induced changes in the acrosomal matrix of some acrosome intact spermatozoa. In both possum and wallaby, freeze-thawing increased the number of spermatozoa with vesiculation of the acromosal matrix. Freeze-thawing disrupted the plasma membrane of spermatozoa but the acromosal membranes remained intact.

Details

  • Original title: The unique stability of the marsupial sperm acrosomal membranes examined by unprotected freeze-thawing and treatment with the detergent Triton X-100.
  • Record ID : 1994-1265
  • Languages: English
  • Source: Reprod. Fertil. Dev. - vol. 5 - n. 1
  • Publication date: 1993
  • Document available for consultation in the library of the IIR headquarters only.

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