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Two freezing cycles ensure interface sterilization by cryosurgery during bone tumour resection.

Author(s) : ROBINSON D., HALPERIN N., NEVO Z.

Type of article: Article

Summary

Cryosurgery utilizing an argon-based system allows bone-tumour interface sterilization, while avoiding the risks of conventional cryosurgery. The study was conducted in order to evaluate the number of freezing cycles required for interface sterilization in cases of aggressive human bone tumours (chondrosarcomas, metastatic carcinomas, and giant cell tumours) affecting long bones. The interface viability was assessed using the XTT method. Quantitative histological evaluation was based on the percentage of live cells divided by total lacunae. One freezing cycle (5 min, -40 °C) reduced tumour viability to about 25% of prefreezing. However, there were still live specimens. Two or three freezing cycles led to complete interface sterilization. The difference between a single freezing cycle and two freezing cycles was significant. The difference between two freezing cycles and three freezing cycles was not significant. Two freezing cycles thus ensure tumour-bone interface sterilization in aggressive human bone tumours.

Details

  • Original title: Two freezing cycles ensure interface sterilization by cryosurgery during bone tumour resection.
  • Record ID : 2002-2156
  • Languages: English
  • Source: Cryobiology - vol. 43 - n. 1
  • Publication date: 2001/08

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