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Ultra-fast vitrification experiment of hamster ovary cells utilizing thin film evaporation.

Number: pap. n. 0498

Author(s) : SU F., FAN Y., XU H., et al.

Summary

Vitrification is one of effective methods for cells cryopreservation. An ultra-fast cooling rate is beneficial to improve the vitrification level of the extra and intracellular solution and increase cell survival rates. Thin film evaporation is an efficient phase change heat transfer mode, and can achieve the ultra-fast cooling rate if it is utilized for cell vitrification. In this paper, a novel ultra-fast cooling method utilizing thin film evaporation was developed, and used for the vitrification of hamster ovary cells. Contrast experiments among this novel method, slow freezing method and open pulled straw vitrification were conducted. The experimental results showed that the novel method achieved a highest cooling rate and cell survival rate than the other two conventional methods. The reason why the novel method achieved the ultra-high cooling rate was mainly that thin film evaporation of liquid nitrogen can avoid the forming of “vapor blanket” and improve the heat transfer coefficient of cell vitrification. The current investigation proved preliminarily the feasibility of this novel cell vitrification method.

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Pages: 9

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Details

  • Original title: Ultra-fast vitrification experiment of hamster ovary cells utilizing thin film evaporation.
  • Record ID : 30025892
  • Languages: English
  • Source: Proceedings of the 25th IIR International Congress of Refrigeration: Montréal , Canada, August 24-30, 2019.
  • Publication date: 2019/08/24
  • DOI: http://dx.doi.org/10.18462/iir.icr.2019.0498

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