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Vitrification media: toxicity, permeability, and dielectric properties.

Author(s) : WUSTEMAN M. C., PEGG D. E., ROBINSON M. P., et al.

Type of article: Article

Summary

The aim of this study was to select a cryoprotectant for use in attempts to preserve tissues and organs by vitrification. The first step was to select a cell line with which to compare the toxicity of a range of commonly used cryoprotectants. An immortal vascular endothelial cell (ECV304) was exposed to vitrifying concentrations of four cryoprotectants: dimethyl sulfoxide (Me2SO; 45% w/w); 2,3 butanediol (BD; 32%); 1,2-propanediol (PD; 45%); and ethanediol (ED; 45%). Three exposure durations (1, 3, and 9 min) and two temperatures (22 and 2-4 °C) were studied. After removal of the cryoprotectant, the ability of the cells to adhere and divide in culture over a 2-day period was measured and expressed as a Cell Survival Index (CSI). There was no measurable loss of cells after exposure to the four cryoprotectants but 3-min exposure to BD, PD, or Me2SO at room temperature completely destroyed the ability of the cells to adhere and divide in culture. In contrast, exposure to all four cryoprotectants at 2-4 °C for up to 9 min permitted the retention of significant cell function. The permeability properties of the cells for these four cryoprotectants was also measured at each temperature. 2-3-propanediol emerged as the most promising vitrifying agent for large tissues and organs.

Details

  • Original title: Vitrification media: toxicity, permeability, and dielectric properties.
  • Record ID : 2003-3015
  • Languages: English
  • Source: Cryobiology - vol. 44 - n. 1
  • Publication date: 2002/02

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