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Loss of mitochondrial function and accumulation of acidic vesicular organelles under low-temperature incubation in HaCaT cells.

Number: pap. n. 0169

Author(s) : NAGAHARA Y., UCHIDA N., MIDORIKAWA M., et al.

Summary

Compared to plants, response to cold stress in animals is regarded as less important; however, in animals, peripheral tissues like skin are sensitive to cold and induce shivering. In this study, we focused on the mitochondrial damage resulting from exposure to low temperature using HaCaT cells as a human epidermal cell model. HaCaT cells were incubated at 4 °C. Mitochondrial membrane potential was observed by a membrane potential indicator dye, JC-1. Reactive oxygen species (ROS) was detected by H2DCFDA and MitoSOX dyes. Acidic vesicular organelles were stained with acridine orange. After incubating the HaCaT cells at 4 °C, the mitochondrial membrane potential decreased and ROS generation increased, leading to cell death. Moreover, accumulation of acidic vesicular organelles was observed and lysosomes were co-localized to the mitochondria. These data suggest that lysosomes degraded the damaged mitochondria for cell survival in cold stress.

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Details

  • Original title: Loss of mitochondrial function and accumulation of acidic vesicular organelles under low-temperature incubation in HaCaT cells.
  • Record ID : 30025891
  • Languages: English
  • Source: Proceedings of the 25th IIR International Congress of Refrigeration: Montréal , Canada, August 24-30, 2019.
  • Publication date: 2019/08/24
  • DOI: http://dx.doi.org/10.18462/iir.icr.2019.00169

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