Share

A TECHNICAL BIAS: DIFFERENCES IN COOLING RATES PREVENT AMPOULES FROM BEING A RELIABLE INDEX OF STEM CELL CRYOPRESERVATION IN LARGE VOLUMES.

Author(s) : DOUAY L., LOPEZ M., GORIN N. C.

Type of article: Article

Summary

THE AUTHORS HAVE STUDIED THE RECOVERY OF HEMATOPOIETIC PROGENITOR CELLS IN AMPOULES AND COMPARED IT TO THE RECOVERY IN STANDARD BAGS. A GOOD PROGENITOR CELL RECOVERY WAS OBSERVED IN ONLY 46% OF FROZEN AMPOULES VERSUS 100% OBSERVED IN FROZEN BAGS. THIS NONOPTIMAL RECOVERY IS RELATED TO THE FREEZING RATE OF 9 K/MIN FOLLOWING THE RELEASE OF FUSION HEAT WHICH OCCURRED IN AMPOULES, WHILE THE FREEZING RATE WAS CON-STANTLY MAINTAINED AT 2 K/MIN IN THE BAGS. THE COOLING CONDITIONS HAVE TO BE CAREFULLY CONTROLLED TO ENSURE THAT THE BAGS AND AMPOULES ARE COOLED UNDER THE SAME CONDITIONS. OTHERWISE, AMPOULES WOULD NOT BE A RELIABLE INDEX OF THE TRUE PROGENITOR CELLS' CRYOPRESERVATION EFFICIENCY IN BAGS.

Details

  • Original title: A TECHNICAL BIAS: DIFFERENCES IN COOLING RATES PREVENT AMPOULES FROM BEING A RELIABLE INDEX OF STEM CELL CRYOPRESERVATION IN LARGE VOLUMES.
  • Record ID : 1987-0377
  • Languages: English
  • Source: Cryobiology - vol. 23 - n. 4
  • Publication date: 1986

Links


See other articles in this issue (4)
See the source

Indexing