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OPTIMIZING CONDITIONS FOR CRYOPRESERVATION OF AN INSECT CELL LINE.

Author(s) : HEACOX A. E., LEOPOLD R. A.

Type of article: Article

Summary

A CELL LINE DERIVED FROM EMBRYOS OF THE COCKROACH BLATTELLA GERMANICA WAS FROZEN TO 77 K (-196 DEG C) UNDER A VARIETY OF CONDITIONS. IT WAS FOUND THAT CELL VIABILITY WAS AFFECTED BY THE COOLING RATE, THE WARMING RATE, THE CONTROLLED COOLING ENDPOINT TEMPERATURE, AND THE TYPE AND CONCENTRATION OF CRYOPROTECTANT. THE BEST SURVIVAL FOR CELLS SUSPENDED IN GRACE'S TISSUE CULTURE MEDIUM WAS OBTAINED WHEN CELLS WERE COOLED AT 1 K/MIN TO AT LEAST 183 K (-90 DEG C) BEFORE BEING PLACEDIN LIQUID NITROGEN AND WARMED AT MORE THAN 900 K/MIN.

Details

  • Original title: OPTIMIZING CONDITIONS FOR CRYOPRESERVATION OF AN INSECT CELL LINE.
  • Record ID : 1985-1294
  • Languages: English
  • Source: Cryobiology - vol. 21 - n. 4
  • Publication date: 1984

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