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CONTROLLED RATE FREEZING OF HUMAN MARROW IN A CONSTANT TEMPERATURE COOLING GRADIENT IN AIR.

Author(s) : FIGUERA A.

Type of article: Article

Summary

A NEW INSTRUMENT WHICH UTILIZES A COMPUTERCONTROLLED FREEZING PLATFORM MOVING IN A CONSTANT AIR TEMPERATURE GRADIENT GENERATED OVER LIQUID NITROGEN (LN) WAS EVALUATED FOR CRYOPRESERVATION OF HUMAN MARROW. MARROWS WERE PLACED HORIZONTALLY ON THE FREEZING PLATFORM WHICH WAS SUSPENDED OVER LN IN A CYLINDRICAL CHAMBER. THE PLATFORM WAS RAISED OR LOWERED TO MAINTAIN A PREDETERMINED FIXED COOLING RATE IN RESPONSE TO TEMPERATURE MONITORED AND RECORDED BY THE COMPUTER FROM A THERMOCOUPLE PLACED AT PLATFORM LEVEL. SEPARATE FREEZING PROGRAMS WERE CREATED FOR DIFFERENT MARROW VOLUMES. RECOVERY AFTER FREEZING AND THAWING WAS THE SAME FOR THE CONSTANT AIR GRADIENT AS FOR A CONVENTIONAL FREEZING INSTRUMENT.

Details

  • Original title: CONTROLLED RATE FREEZING OF HUMAN MARROW IN A CONSTANT TEMPERATURE COOLING GRADIENT IN AIR.
  • Record ID : 1987-0379
  • Languages: English
  • Source: Cryobiology - vol. 23 - n. 5
  • Publication date: 1986

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