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AN HYPOTHESIS FOR SURVIVAL OF SPERMATOZOA VIA ENCAPSULATION DURING PLANE FRONT FREEZING.

Author(s) : BROWER W. E.

Type of article: Article

Summary

ENCAPSULATION OF CELLS BY A PLANE FRONT ICE INTERFACE SHOULD RESULT IN THREE REGIONS OF CELL SURVIVAL IN THE SAMPLE: AN INITIAL REGION OF CELL DEATH DUE TO INTRACELLULAR ICE FORMATION, A FINAL REGION OF CELL DEATH DUE TO OVEREXPOSURE TO HYPERTONIC INTERCELLULAR LIQUID, AND AN INTERMEDIATE REGION OF CELL SURVIVAL, WHERE NEITHER DAMAGE MECHANISM HAS OPERATED TO A LETHAL LEVEL. AN ADVANTAGE OF PLANE FRONT FREEZING OVER DENDRITIC FREEZING IS THAT THE REGIONS OF CELL SURVIVAL AND DEATH SHOULD BE GEOMETRICALLY SEPARATE IN THE SAMPLE, RATHER THAN MIXED AT THE DENDRITIC MICROSTRUCTURAL LEVEL, AS IS THE CASE FOR DENDRITIC FREEZING.

Details

  • Original title: AN HYPOTHESIS FOR SURVIVAL OF SPERMATOZOA VIA ENCAPSULATION DURING PLANE FRONT FREEZING.
  • Record ID : 1982-0641
  • Languages: English
  • Source: Cryobiology - vol. 18 - n. 3
  • Publication date: 1981/06

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