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Application of the very low temperatures for the preservation of rare plant seeds of Ukraine.

Number: pap. n. 004

Author(s) : ARAPETYAN E., YU U.

Summary

Alpine aster is included in the Red Data Book of Ukraine and spread in the Ukrainian Carpathian Mountains. Survival of seeds of Alpine aster in liquid nitrogen was studied. The seeds were immersed into Dewar vessel filled with liquid nitrogen (-196°C). Cryoprotectants were not used. After a month of cryopreservation the seeds did not lose their viability. In investigated species no significant difference in the final germination percentages between experiment and control variants was found. The storage in liquid nitrogen within a month period did not affect the further growth and normal development of the cryopreserved object. Plantlets, which were formed as a result of germination tests were planted in the living collections of the Botanical Garden. Growth and development of these plants were in the normal range within three years. Flowering began on the third year.

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Pages: 150-153

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Details

  • Original title: Application of the very low temperatures for the preservation of rare plant seeds of Ukraine.
  • Record ID : 30005947
  • Languages: English
  • Source: Cryogenics 2012. Proceedings of the 12th IIR International Conference: Dresden, Germany, September 11-14, 2012.
  • Publication date: 2012/09/11

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